Anti-CTIP RBBP8 Antibody

CtIP antibody

Boster Bio Anti-CTIP RBBP8 Antibody (Catalog# A02076). Tested in IHC-P, IF (paraffin), WB, ELISA applications. This antibody reacts with Human, Mouse.

Product Info Summary

SKU: A02076
Size: 100ul
Reactive Species: Human, Mouse
Host: Rabbit
Application: ELISA, IHC-P, WB, IF (paraffin)

Product Name

Anti-CTIP RBBP8 Antibody

View all CtIP Antibodies

SKU/Catalog Number

A02076

Size

100ul

Form

Liquid

Description

Boster Bio Anti-CTIP RBBP8 Antibody (Catalog# A02076). Tested in IHC-P, IF (paraffin), WB, ELISA applications. This antibody reacts with Human, Mouse.

Storage & Handling

Store at -20°C for one year. For short-term storage and frequent use, store at 4°C for up to one month. Avoid repeated freeze-thaw cycles.

Cite This Product

Anti-CTIP RBBP8 Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # A02076)

Host

Rabbit

Contents

PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide.

Clonality

Polyclonal

Clone Number

7A7

Isotype

Rabbit IgG

Immunogen

A peptide derived from human CTIP. Immunogen sequence location: 293-342

*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.

Cross-reactivity

No cross reactivity with other proteins.

Reactive Species

A02076 is reactive to RBBP8 in Human, Mouse

Applications

A02076 is guaranteed for ELISA, IHC-P, WB, IF (paraffin) Boster Guarantee

Observed Molecular Weight

72 kDa

Calculated molecular weight

101.942kDa

Background of CtIP

Integrin alpha 1 (ITGA1) chain associates with the beta 1 (ITGB1) chain to form a heterodimer that functions as a dual laminin/collagen receptor in neural cells and hematopoietic cells. ITGA1 has a 206-amino acid I domain in its N-terminal half, followed by 3 divalent cation-binding sites and a C-terminal transmembrane domain with a short cytoplasmic tail. It also has 28 potential N-glycosylation sites. Human ITGA1 was expressed in a mouse fibroblast cell line as a 180-kD protein. ITGA1 is involved in the early remodeling of osteoarthritic cartilage and plays an essential role in the regulation of mesenchymal stem cell proliferation and cartilage production. It also plays an essential role in the regulation of MSC proliferation and cartilage production.

Antibody Validation

Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.

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Reconsitution

Add 0.2ml of distilled water will yield a concentration of 500ug/ml.

Assay Dilutions Recommendation

The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.

Western Blot, 1:500-2000
Immunohistochemistry, 1:100 - 1:300
ELISA, 1:40000
Not yet tested in other applications.

Validation Images & Assay Conditions

Gene/Protein Information For RBBP8 (Source: Uniprot.org, NCBI)

Gene Name

RBBP8

Full Name

DNA endonuclease RBBP8

Weight

101.942kDa

Superfamily

COM1/SAE2/CtIP family

Alternative Names

CtBP-interacting protein; CtIP; DNA endonuclease RBBP8; EC 3.1; RBBP-8; retinoblastoma binding protein 8; Retinoblastoma-binding protein 8; Retinoblastoma-interacting protein and myosin-like; RIMSAE2; Sporulation in the absence of SPO11 protein 2 homolog RBBP8 COM1, CTIP, JWDS, RIM, SAE2, SCKL2 RB binding protein 8, endonuclease DNA endonuclease RBBP8|CTBP-interacting protein|RBBP-8|retinoblastoma binding protein 8|sporulation in the absence of SPO11 protein 2 homolog

*If product is indicated to react with multiple species, protein info is based on the gene entry specified above in "Species".

For more info on RBBP8, check out the RBBP8 Infographic

RBBP8 infographic

We have 30,000+ of these available, one for each gene! Check them out.

In this infographic, you will see the following information for RBBP8: database IDs, superfamily, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post-translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact [email protected].

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5 Customer Q&As for Anti-CTIP RBBP8 Antibody

Question

See below the WB image, lot number and protocol we used for testis using anti-CTIP antibody A02076. Please let me know if you require anything else.

Verified Customer

Verified customer

Asked: 2020-02-25

Answer

Thank you very much for the data. Our lab team are working to resolve this as quickly as possible, and we appreciate your patience and understanding! You have provided everything we needed. Please let me know if there is anything you need in the meantime.

Boster Scientific Support

Answered: 2020-02-25

Question

you antibody to test anti-CTIP antibody A02076 on mouse testis for research purposes, then I may be interested in using anti-CTIP antibody A02076 for diagnostic purposes as well. Is the antibody suitable for diagnostic purposes?

Verified Customer

Verified customer

Asked: 2019-10-14

Answer

The products we sell, including anti-CTIP antibody A02076, are only intended for research use. They would not be suitable for use in diagnostic work. If you have the means to develop a product into diagnostic use, and are interested in collaborating with us and develop our product into an IVD product, please contact us for more discussions.

Boster Scientific Support

Answered: 2019-10-14

Question

We are currently using anti-CTIP antibody A02076 for mouse tissue, and we are content with the IHC results. The species of reactivity given in the datasheet says human, mouse. Is it true that the antibody can work on bovine tissues as well?

J. Moore

Verified customer

Asked: 2019-01-03

Answer

The anti-CTIP antibody (A02076) has not been tested for cross reactivity specifically with bovine tissues, though there is a good chance of cross reactivity. We have an innovator award program that if you test this antibody and show it works in bovine you can get your next antibody for free. Please contact me if I can help you with anything.

Boster Scientific Support

Answered: 2019-01-03

Question

I appreciate helping with my inquiry over the phone. Here are the WB image, lot number and protocol we used for testis using anti-CTIP antibody A02076. Let me know if you need anything else.

Verified Customer

Verified customer

Asked: 2018-07-16

Answer

We appreciate the data. You have provided everything we needed. Our lab team are working to resolve your inquiry as quickly as possible, and we appreciate your patience and understanding! Please let me know if there is anything you need in the meantime.

Boster Scientific Support

Answered: 2018-07-16

Question

I see that the anti-CTIP antibody A02076 works with IHC, what is the protocol used to produce the result images on the product page?

D. Jackson

Verified customer

Asked: 2013-09-25

Answer

You can find protocols for IHC on the "support/technical resources" section of our navigation menu. If you have any further questions, please send an email to [email protected]

Boster Scientific Support

Answered: 2013-09-25

Order DetailsPrice
A02076

100 μL

$335

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*Sample sizes are prepared on demand and will take extra lead time. (cannot be conjugated)
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